Coding

Part:BBa_K2114017:Design

Designed by: Wladislaw Stroukov   Group: iGEM16_Freiburg   (2016-10-10)


aGFPnano_HA_aHelix_cgeA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 681
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Introduction of HA epitope tag and alpha helical linker by primer extensions and PCR.


Source

The cgeA gene comes from the genome of B. subtilis. The anti-GFP nanobody is derived from camelid heavy-chain antibodies described by Kubala et al. in 2010. [Ref]

References